A sensitive fluorometric sensor for Ag⁺ based on the hybridization chain reaction coupled with a glucose oxidase dual-signal amplification strategy.

ABSTRACT: In this work, an efficient and sensitive fluorometric sensor was developed to detect silver ions (Ag⁺). It is based on the cytosine-Ag⁺-cytosine (C-Ag⁺-C) structure via a dual-signal amplification strategy using glucose oxidase (GOx) and the hybridization chain reaction (HCR). A silver-coated glass slide (SCGS) acts as an ideal material for separation. Cytosine rich (C-rich) capture DNA (C-DNA) assembled themselves on the SCGS via Ag-S bonds and hybridized with signal DNA (S-DNA) to trigger the HCR. With specific base-pairing, the S-DNA and HCR products bind on the SCGS. Then, the GOx-biotin-streptavidin (SA) complexes bind to the HCR products through SA-biotin interactions. Owing to the formation of a particular C-Ag⁺-C structure b

SUBMITTER: Li Y 

PROVIDER: S-EPMC9055297 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature