Preparative separation of mangiferin glycosides by high speed counter current chromatography and comparison of their antioxidant and antitumor activities.

ABSTRACT: Mangiferin, a xanthonoid with various bioactivities. The low solubility of mangiferin limits the use in pharmacological fields. In this study, high-speed counter-current chromatography (HSCCC) was used to separate and purify mangiferin glycosides from the crude sample after enzymatic glycosylation of mangiferin. Two fructosyl mangiferin were successfully purified by HSCCC with a two-phase-solvent system composed of n-butanol-methanol-water (6 : 1 : 6, v/v). A total of 18 mg of mangiferin (I), 73 mg of β-d-fructofuranosyl-(2 → 6)-mangiferin (II), and 58 mg of β-d-difructofuranosyl-(2 → 6)-mangiferin (III) were obtained in one-step separation from 150 mg of the crude sample with purities of 99.2%, 98.7% and 98.9%, respectively. The chemical structures were identified by HRMS, ^1</

SUBMITTER: Xu T 

PROVIDER: S-EPMC9055302 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature