Evaluation of SARS-CoV-2 concentrations in wastewater and river water samples.

ABSTRACT: There are only a few established methods to determine the concentration of encapsulated viruses, such as SARS-CoV-2, in water matrices, limiting the application of wastewater-based epidemiology (WBE)-an important tool for public health research. The present study compared four methods that are commonly used to concentrate non-encapsulated enteric viruses for determining SARS-CoV-2 concentration in wastewater and wastewater-enriched river water samples. The four methods tested were electronegative membrane with Mg⁺² addition, aluminum hydroxide-based precipitation, polyethylene glycol (PEG) 8000 precipitation, and ultrafiltration (with porosity of 10 and 50 kDa). Prior to the concentration step, filtration or centrifugation was performed to remove suspended particles from the sam

SUBMITTER: Fonseca MS 

PROVIDER: S-EPMC9055419 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature