Repressed miR-34a Expression Dictates the Cell Fate to Corneal Endothelium Failure.

ABSTRACT:

Purpose

To reveal the mechanism triggering the functional disparity between degenerated and non-degenerated corneal endothelium cells in the water efflux from corneal stroma to the anterior chamber.

Methods

The varied levels of the microRNA (miR)-34, miR-378, and miR-146 family in human corneal endothelium and cultured cells thereof were investigated using 3D-Gene Human miRNA Oligo Chips. Concomitantly, CD44, p53, c-Myc, matrix metalloprotease (MMP)-2 expression, and Ras homolog gene family member A (Rho A) activity was correlated to the expression intensities of these microRNAs, partly complemented with their altered expression levels with the transfection of the corresponding mimics and inhibitors. The levels of miRs were further associated with intracellular pH (pHi) and

SUBMITTER: Hamuro J 

PROVIDER: S-EPMC9055560 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature