Genetic control of violacein biosynthesis to enable a pigment-based whole-cell lead biosensor.

ABSTRACT: Environmental risks continue to grow due to heavy metal contamination caused by anthropogenic activities. Accumulation of harmful quantities of lead poses a threat to aquatic organisms, plants, and human beings. Whole-cell biosensors, which can proliferate independently, can detect the bioavailable fraction to assess the effect of target heavy metal on the environmental ecosystem. In this study, the biosynthesis pathway of violacein was heterogeneously constructed under the control of the T7 lac promoter in E. coli. A dose-response relationship existed between the inducer and the production of violacein. The biosynthesis pathway of violacein was finally engineered under the regulation of Pb(ii)-dependent metalloregulator PbrR to assemble Pb(ii)-inducible whole-cell biosensor. It per

SUBMITTER: Hui CY 

PROVIDER: S-EPMC9055639 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature