Lignin nanoparticles are renewable and functional platforms for the concanavalin a oriented immobilization of glucose oxidase-peroxidase in cascade bio-sensing.

ABSTRACT: Lignin nanoparticles (LNPs) acted as a renewable and efficient platform for the immobilization of horseradish peroxidase (HRP) and glucose oxidase (GOX) by a layer by layer procedure. The use of concanavalin A as a molecular spacer ensured the correct orientation and distance between the two enzymes as confirmed by Förster resonance energy transfer measurement. Layers with different chemo-physical properties tuned in a different way the activity and kinetic parameters of the enzymatic cascade, with cationic lignin performing as the best polyelectrolyte in the retention of the optimal Con A aggregation state. Electrochemical properties, temperature and pH stability, and reusability of the novel systems have been studied, as well as their capacity to perform as colorimetric biosensors in the

SUBMITTER: Capecchi E 

PROVIDER: S-EPMC9055843 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature