The selection of highly specific and selective aptamers using modified SELEX and their use in process analytical techniques for Lucentis bioproduction.

ABSTRACT: Aptamers for Lucentis were selected using 10 rounds of a modified and highly stringent SELEX process. Affinity column chromatography was used for the binding, partitioning, and elution steps, and the regeneration of ssDNA was performed via asymmetric PCR in the SELEX process. The interaction of aptamers with Lucentis was studied by means of the HADDOCK web server docking program. In addition, the secondary structures of aptamers were interrogated using the mfold web server to check common regions responsible for better affinity towards Lucentis. The two best aptamers for Lucentis (aptamers 1 and 25) were found to have dissociation constant (K _d) values between 23 and 35 nM by means of thermofluorimetric and non-faradaic impedance spectroscopy (NFIS) analysis. The l

SUBMITTER: Bhardwaj T 

PROVIDER: S-EPMC9055848 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature