Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit.

ABSTRACT:

Objectives

The rats are crucial animal models for the basic medical researches. Rat embryonic stem cells (ESCs), which are widely studied, can self-renew and exhibit pluripotency in long-term culture, but the mechanism underlying how they exit pluripotency remains obscure. To investigate the key modulators on pluripotency exiting in rat ESCs, we perform genome-wide screening using a unique rat haploid system.

Materials and methods

Rat haploid ESCs (haESCs) enable advances in the discovery of unknown functional genes owing to their homozygous and pluripotent characteristics. REX1 is a sensitive marker for the naïve pluripotency that is often utilized to monitor pluripotency exit, thus rat haESCs carrying a Rex1-GFP reporter are used for genetic screening. Genome-wide mutation

SUBMITTER: Xu M 

PROVIDER: S-EPMC9055895 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature