Retinal organoids derived from rhesus macaque iPSCs undergo accelerated differentiation compared to human stem cells.

ABSTRACT:

Purpose

To compare the timing and efficiency of the development of Macaca mulatta, a nonhuman primate (NHP), induced pluripotent stem cell (rhiPSC) derived retinal organoids to those derived from human embryonic stem cells (hESCs).

Results

Generation of retinal organoids was achieved from both human and several NHP pluripotent stem cell lines. All rhiPSC lines resulted in retinal differentiation with the formation of optic vesicle-like structures similar to what has been observed in hESC retinal organoids. NHP retinal organoids had laminated structure and were composed of mature retinal cell types including cone and rod photoreceptors. Single-cell RNA sequencing was conducted at two time points; this allowed identification of cell types and developmental trajectory character

SUBMITTER: Jacobo Lopez A 

PROVIDER: S-EPMC9055909 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature