Purification and characterization of a thaumatin-like protein-1 with polyphenol oxidase activity found in Prunus mume.

ABSTRACT: Thaumatin-like protein-1 (TLP-1), a protein displaying high polyphenol oxidase (PPO) action and a member of the pathogenesis-related (PR) protein family, has a considerable influence on the enzymatic browning of Prunus mume (Chinese plum). In this assay, TLP-1 was identified and extracted from Prunus mume to investigate the protein's properties and better understand its contribution to the fruit's browning during storage or processing. The extracted TLP-1 was purified to apparent homogeneity using a procedure involving citrate phosphate buffer solution (CPBS) extraction, (NH₄)₂SO₄ precipitation, dialysis in a cellulose bag, and ion exchange chromatography using a DEAE Sepharose Fast Flow column, while a Sephadex G-75 column was employed to fac

SUBMITTER: Ma Y 

PROVIDER: S-EPMC9055924 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature