Dataset Information

Synthesis of magnetic core-shell Fe₃O₄@PDA@Cu-MOFs composites for enrichment of microcystin-LR by MALDI-TOF MS analysis.

ABSTRACT: Microcystin-LR (MC-LR) is a toxin released from cyanobacteria in eutrophicated water. MC-LR is the most abundant and the most toxic among microcystins. In this work, core-shell structured copper-based magnetic metal-organic framework (Fe₃O₄@PDA@Cu-MOFs) composites were synthesized via a solvothermal reaction and a sol-gel method. The Fe₃O₄@PDA@Cu-MOFs composites showed ultra-high surface area, strong magnetic response and outstanding hydrophilicity. The Fe₃O₄@PDA@Cu-MOFs composites combined with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) were used to analyse the content of MC-LR in real water samples. Under the optimised conditions, our proposed method exhibited good linearity within a concentration range of 0.05-4 μg L^-1 and good detection even at low limits (0.015 μg L^-1). The method was also successfully applied to analyse traces of MC-LR with quantitative recoveries for the real water samples in the range from 98.67% to 106.15%. Furthermore, it was characterized by high sensitivity, short operation time, being environmental friendly and having the ability to analyse other pollutants in the environment.

SUBMITTER: Li Z

PROVIDER: S-EPMC9055938 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Synthesis of magnetic core-shell Fe3O4@PDA@Cu-MOFs composites for enrichment of microcystin-LR by MALDI-TOF MS analysis.

Li Zhijian Z Gong Congcong C Huo Panpan P Deng Chunhui C Pu Shouzhi S

RSC advances 20200805 49

Microcystin-LR (MC-LR) is a toxin released from cyanobacteria in eutrophicated water. MC-LR is the most abundant and the most toxic among microcystins. In this work, core-shell structured copper-based magnetic metal-organic framework (Fe3O4@PDA@Cu-MOFs) composites were synthesized via a solvothermal reaction and a sol-gel method. The Fe3O4@PDA@Cu-MOFs composites showed ultra-high surface area, strong magnetic response and outstanding hydrophilic ...[more]

PMID: 35521136

Similar Datasets

Project description:BackgroundSerotyping of Streptococcus pneumoniae is important for monitoring of vaccine impact. Unfortunately, conventional and molecular serotyping is expensive and technically demanding. This study aimed to determine the ability of matrix-assisted laser desorption-ionisation time-of-flight (MALDI-TOF) mass spectrometry to discriminate between pneumococcal serotypes and genotypes (defined by global pneumococcal sequence cluster, GPSC). In this study, MALDI-TOF mass spectra were generated for a diverse panel of whole genome sequenced pneumococcal isolates using the bioMerieux VITEK MS in clinical diagnostic (IVD) mode. Discriminatory mass peaks were identified and hierarchical clustering was performed to visually assess discriminatory ability. Random forest and classification and regression tree (CART) algorithms were used to formally determine how well serotypes and genotypes were identified by MALDI-TOF mass spectrum.ResultsOne hundred and ninety-nine pneumococci, comprising 16 serotypes and non-typeable isolates from 46 GPSC, were analysed. In the primary experiment, hierarchical clustering revealed poor congruence between MALDI-TOF mass spectrum and serotype. The correct serotype was identified from MALDI-TOF mass spectrum in just 14.6% (random forest) or 35.4% (CART) of 130 isolates. Restricting the dataset to the nine dominant GPSC (61 isolates / 13 serotypes), discriminatory ability improved slightly: the correct serotype was identified in 21.3% (random forest) and 41.0% (CART). Finally, analysis of 69 isolates of three dominant serotype-genotype pairs (6B-GPSC1, 19F-GPSC23, 23F-GPSC624) resulted in the correct serotype identification in 81.1% (random forest) and 94.2% (CART) of isolates.ConclusionsThis work suggests that MALDI-TOF is not a useful technique for determination of pneumococcal serotype. MALDI-TOF mass spectra appear more associated with isolate genotype, which may still have utility for future pneumococcal surveillance activities.

Dataset Information

Synthesis of magnetic core-shell Fe₃O₄@PDA@Cu-MOFs composites for enrichment of microcystin-LR by MALDI-TOF MS analysis.

Publications

Synthesis of magnetic core-shell Fe<sub>3</sub>O<sub>4</sub>@PDA@Cu-MOFs composites for enrichment of microcystin-LR by MALDI-TOF MS analysis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets