Extraction and purification of ε-poly-l-lysine from fermentation broth using an ethanol/ammonium sulfate aqueous two-phase system combined with ultrafiltration.

ABSTRACT: ε-Poly-l-lysine (ε-PL) serves as a natural food preservative and is manufactured mainly by extraction from microbial fermentation broth using ion-exchange chromatography. In order to develop an alternative purification strategy, an environmentally friendly alcohol/salt aqueous two-phase system (ATPS) was explored in this study for ε-PL extraction. A study of the separation of ε-PL in different alcohol/salt systems showed that ethanol/ammonium sulfate ATPS exhibited the highest ε-PL partition coefficient and recovery ratio. Based on the phase diagram, the effect of phase composition on partition, and the removal of pigment and protein, an ATPS that was composed of 20% (w/w) ethanol and 20% (w/w) ammonium sulfate, with a feedstock at pH 9.5, was developed to extract ε-PL from the fermentatio

SUBMITTER: Chen X 

PROVIDER: S-EPMC9055990 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature