Easily fabricated monolithic fluoropolymer chips for sensitive long-term absorbance measurement in droplet microfluidics.

ABSTRACT: Maintaining a hydrophobic channel surface is critical to ensuring long-term stable flow in droplet microfluidics. Monolithic fluoropolymer chips ensure robust and reliable droplet flow as their native fluorous surfaces naturally preferentially wet fluorocarbon oils and do not deteriorate over time. Their fabrication, however, typically requires expensive heated hydraulic presses that make them inaccessible to many laboratories. Here we describe a method for micropatterning and bonding monolithic fluoropolymer flow cells from a commercially available melt-processable fluoropolymer, Dyneon THV 500GZ, that only requires a standard laboratory oven. Using this technique, we demonstrate the formation of complex microstructures, specifically the fabrication of sensitive absorbance flow cells for

SUBMITTER: Nightingale AM 

PROVIDER: S-EPMC9056331 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature