An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection.

ABSTRACT: Nucleic acids have special ability to organize themselves into various non-canonical structures, including a four-stranded DNA structure termed G-quadruplex (G4) that has been utilized for diagnostic and therapeutic applications. Herein, we report the ability of G4 to distinguish dengue virus (DENV) based on its serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) using a split G4-hemin DNAzyme configuration. In this system, two separate G-rich oligonucleotides are brought together upon target DNA strand hybridization to form a three-way junction architecture, allowing the formation of a G4 structure. The G4 formation in complexation with hemin can thus provide a signal readout by generating a DNAzyme that is able to catalyze H₂O₂-mediated oxidation of 2,2'-azino-bis(3-ethyl

SUBMITTER: Ida J 

PROVIDER: S-EPMC9056686 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature