Evaluation of the site-unspecified peptide identification method for proteolytic peptide mapping.

ABSTRACT: Proteases are widely used in the food industry to hydrolyze proteins and prepare bioactive peptides. Peptide mapping identification supports the application of proteases in the food industry. The site-specified peptide identification method, which was developed for site-specific proteases like trypsin, is relatively mature and reliable but cannot be applied using most industrial proteases with weak site specificity. To address this issue, the performance and reliability of the site-unspecified peptide identification method should be investigated and evaluated. In this study, tryptic hydrolysates of a single protein and a protein mixture were used to evaluate the site-unspecified identification method. The species origin of the hydrolyzed proteins was not specified in a database search, mea

SUBMITTER: Wang HB 

PROVIDER: S-EPMC9057140 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature