Assessing the compatibility of primary human hepatocyte culture within porous silk sponges.

ABSTRACT: Donor organ shortages have prompted the development of alternative implantable human liver tissues for patients suffering from end-stage liver failure. Purified silk proteins provide desirable features for generating implantable tissues, including sustainable sourcing from insects/arachnids, biocompatibility, tunable mechanical properties and degradation rates, and low immunogenicity upon implantation. While different cell types were previously cultured for weeks within silk-based scaffolds, it remains unclear whether such scaffolds can be used to culture primary human hepatocytes (PHH) isolated from livers. Therefore, here we assessed the compatibility of PHH culture within porous silk scaffolds that enable diffusion of oxygen/nutrients through the pores. We found that incorporation of ty

SUBMITTER: Kukla DA 

PROVIDER: S-EPMC9057238 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature