Fecal metagenomics and metabolomics reveal gut microbial changes after bariatric surgery.

ABSTRACT:

Background

Evidence from longitudinal patient studies regarding gut microbial changes after bariatric surgery is limited.

Objective

To examine intraindividual changes in fecal microbiome and metabolites among patients undergoing Roux-en-Y gastric bypass or vertical sleeve gastrectomy.

Setting

Observational study.

Methods

Twenty patients were enrolled and provided stool samples before and 1 week, 1 month, and/or 3 months after surgery. Shallow shotgun metagenomics and untargeted fecal metabolomics were performed. Zero-inflated generalized additive models and linear mixed models were applied to identify fecal microbiome and metabolites changes, with adjustment for potential confounders and correction for multiple testing.

Results

We enrolled 16 women and 4

SUBMITTER: Yu D 

PROVIDER: S-EPMC9057387 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature