Determination of berberine in Rhizoma coptidis using a β-cyclodextrin-sensitized fluorescence method.

ABSTRACT: In this work, a method for the determination of berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology is established. Berberine is the main extract of Rhizoma coptidis, a medicinal material, which causes an envelope reaction with β-cyclodextrin to generate fluorescence sensitization. In the environment of its own aqueous extract, with 0.0065 mol L^-1 of β-cyclodextrin, a fluorescence excitation wavelength (λ _ex) of 345 nm and an emission wavelength (λ _em) of 540 nm were selected to avoid interference from other distractors. The fluorescent sensor for the detection of berberine exhibits a low limit of detection (3.59 × 10^-9 mol L^-1) and a wide linear range from 2.7 × 10^-7 mol L

SUBMITTER: Zheng YH 

PROVIDER: S-EPMC9057487 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature