Dissecting the molecular control of immune cell accumulation in the inflamed joint.

ABSTRACT: Mechanisms governing entry and exit of immune cells into and out of inflamed joints remain poorly understood. We sought herein to identify the key molecular pathways regulating such migration. Using murine models of inflammation in conjunction with mice expressing a photoconvertible fluorescent protein, we characterized the migration of cells from joints to draining lymph nodes and performed RNA-Seq analysis on isolated cells, identifying genes associated with migration and retention. We further refined the gene list to those specific for joint inflammation. RNA-Seq data revealed pathways and genes previously highlighted as characteristic of rheumatoid arthritis in patient studies, validating the methodology. Focusing on pathways associated with cell migration, adhesion, and movement, we i

SUBMITTER: Prendergast CT 

PROVIDER: S-EPMC9057592 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature