Matrix Hyaluronic Acid and Hypoxia Influence a CD133⁺ Subset of Patient-Derived Glioblastoma Cells.

ABSTRACT: Glioblastoma (GBM) displays diffusive invasion throughout the brain microenvironment, which is partially responsible for its short median survival rate (<15 months). Stem-like subpopulations (GBM stem-like cells, GSCs) are believed to play a central role in therapeutic resistance and poor patient prognosis. Given the extensive tissue remodeling and processes such as vessel co-option and regression that occur in the tumor microenvironment, it is essential to understand the role of metabolic constraint such as hypoxia on GBM cell populations. This work describes the use of a multidimensional gelatin hydrogel to culture patient-derived GBM cells, to evaluate the influence of hypoxia and the inclusion brain-mimetic hyaluronic acid on the relative activity of GSCs versus overall GBM cells. Nota

SUBMITTER: Chen JE 

PROVIDER: S-EPMC9057908 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature