An antibody-based proximity labeling protocol to identify biotinylated interactors of SARS-CoV-2.

ABSTRACT: Elucidating the molecular interactions between virus and host is fundamental to understanding the mechanism of viral pathogenesis. Here, we present a protocol to screen SARS-CoV-2 protein interactors using an antibody-based TurboID proximity labeling approach. This technique directly identifies biotinylated peptides labeled by the TurboID-tagged viral proteins. We describe the steps to prepare biotinylated peptide samples for mass spectrometry analysis and a stringent workflow to identify biotinylated high-confidence interactors of the virus by filtering out non-specific co-purified proteins. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).

SUBMITTER: Shang L 

PROVIDER: S-EPMC9057979 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature