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Use of ammonium salts or binary mixtures derived from amino acids, glycine betaine, choline and indole-3-butyric acid as plant regulators.

ABSTRACT: A simple, efficient, and environmentally friendly synthesis method for bioproducts based on indole-3-butyric acid and amino acids, glycine betaine or choline has been developed. Spectral analysis and molecular calculations were used to determine whether the products were ammonium salts or binary mixtures. Moreover, it was observed that the ammonium salts degraded more rapidly than the binary mixtures when exposed to light. The structures of the products significantly impacted their thermal stability and phase transitions. Biological studies clearly showed that the synthesized products were more effective than a reference commercial preparation as a rooting agent and have significant potential as new biologically active agents with low environmental impact.

SUBMITTER: Kaczmarek DK

PROVIDER: S-EPMC9058124 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Use of ammonium salts or binary mixtures derived from amino acids, glycine betaine, choline and indole-3-butyric acid as plant regulators.

Kaczmarek Damian Krystian DK Parus Anna A Łożyński Marek M Pernak Juliusz J

RSC advances 20201126 70

A simple, efficient, and environmentally friendly synthesis method for bioproducts based on indole-3-butyric acid and amino acids, glycine betaine or choline has been developed. Spectral analysis and molecular calculations were used to determine whether the products were ammonium salts or binary mixtures. Moreover, it was observed that the ammonium salts degraded more rapidly than the binary mixtures when exposed to light. The structures of the products significantly impacted their thermal stabi ...[more]

PMID: 35514883

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Project description:Fluctuations in osmolarity are one of the most prevalent stresses to which bacteria must adapt, both hypo- and hyperosmotic conditions. Most bacteria cope with high osmolarity by accumulating compatible solutes (osmolytes) in the cytoplasm to maintain the turgor pressure of the cell. Vibrio parahaemolyticus, a halophile, utilizes at least six compatible solute transporters for the uptake of osmolytes: two ABC family ProU transporters and four betaine-carnitine-choline transporter (BCCT) family transporters. The full range of compatible solutes transported by this species has yet to be determined. Using an osmolyte phenotypic microarray plate for growth analyses, we expanded the known osmolytes used by V. parahaemolyticus to include N,N-dimethylglycine (DMG), among others. Growth pattern analysis of four triple-bccT mutants, possessing only one functional BCCT, indicated that BccT1 (VP1456), BccT2 (VP1723), and BccT3 (VP1905) transported DMG. BccT1 was unusual in that it could take up both compounds with methylated head groups (glycine betaine [GB], choline, and DMG) and cyclic compounds (ectoine and proline). Bioinformatics analysis identified the four coordinating amino acid residues for GB in the BccT1 protein. In silico modeling analysis demonstrated that GB, DMG, and ectoine docked in the same binding pocket in BccT1. Using site-directed mutagenesis, we showed that a strain with all four residues mutated resulted in the loss of uptake of GB, DMG, and ectoine. We showed that three of the four residues were essential for ectoine uptake, whereas only one of the residues was important for GB uptake. Overall, we have demonstrated that DMG is a highly effective compatible solute for Vibrio species and have elucidated the amino acid residues in BccT1 that are important for the coordination of GB, DMG, and ectoine transport.IMPORTANCEVibrio parahaemolyticus possesses at least six osmolyte transporters, which allow the bacterium to adapt to high-salinity conditions. In this study, we identified several additional osmolytes that were utilized by V. parahaemolyticus We demonstrated that the compound DMG, which is present in the marine environment, was a highly effective osmolyte for Vibrio species. We determined that DMG is transported via BCCT family carriers, which have not been shown previously to take up this compound. BccT1 was a carrier for GB, DMG, and ectoine, and we identified the amino acid residues essential for the coordination of these compounds. The data suggest that for BccT1, GB is more easily accommodated than ectoine in the transporter binding pocket.

Dataset Information

Use of ammonium salts or binary mixtures derived from amino acids, glycine betaine, choline and indole-3-butyric acid as plant regulators.

Publications

Use of ammonium salts or binary mixtures derived from amino acids, glycine betaine, choline and indole-3-butyric acid as plant regulators.

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