Controlling ion channel function with renewable recombinant antibodies.

ABSTRACT: Selective ion channel modulators play a critical role in physiology in defining the contribution of specific ion channels to physiological function and as proof of concept for novel therapeutic strategies. Antibodies are valuable research tools that have broad uses including defining the expression and localization of ion channels in native tissue, and capturing ion channel proteins for subsequent analyses. In this review, we detail how renewable and recombinant antibodies can be used to control ion channel function. We describe the different forms of renewable and recombinant antibodies that have been used and the mechanisms by which they modulate ion channel function. We highlight the use of recombinant antibodies that are expressed intracellularly (intrabodies) as genetically encoded to

SUBMITTER: Colecraft HM 

PROVIDER: S-EPMC9058206 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature