Low-cost, open-source cell culture chamber for regulating physiologic oxygen levels.

ABSTRACT: The physiological oxygen levels for several mammalian cell types in vivo are considered to be hypoxic (low oxygen tension), but the vast majority of in vitro mammalian cell culture is conducted at atmospheric oxygen levels of around 21%. In order to understand the impact of low oxygen environments on cells, oxygen levels need to be regulated during in vitro culture. Two common methods for simulating a hypoxic environment are through the regulation of gas composition or chemical induction. Chemically mimicking hypoxia can have adverse effects such as reducing cell viability, making oxygen regulation in cell culture chambers crucial for long-term culture. However, oxygen-regulating cell culture incubators and commercial hypoxia chambers may not always be a viable option

SUBMITTER: Marchus CRN 

PROVIDER: S-EPMC9058583 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature