Circular and linear: a tale of aptamer selection for the activation of SIRT1 to induce death in cancer cells.

ABSTRACT: It is a challenge to select the right target to treat conditions without affecting non-diseased cells. Cancer belongs to the top 10 causes of death in the world and it remains difficult to treat. Amongst cancer emerging targets, silent information regulator 1 (SIRT1) - a histone deacetylase - has shown many roles in cancer, ageing and metabolism. Here we report novel SIRT1 ligands that bind and modulate the activity of SIRT1 within cells and enhance its enzymatic activity. We developed a modified aptamer capable of binding to and forming a complex with SIRT1. Our ligands are aptamers, they can be made of DNA or RNA oligonucleotides, their binding domain can recognise a target with very high affinity and specificity. We used the systematic evolution of ligands by exponential enrichment (SEL

SUBMITTER: Al-Sudani B 

PROVIDER: S-EPMC9058605 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature