Liver Endothelial Heg Regulates Vascular/Biliary Network Patterning and Metabolic Zonation Via Wnt Signaling.

ABSTRACT:

Background & aims

The liver has complex interconnecting blood vessel and biliary networks; however, how the vascular and biliary network form and regulate each other and liver function are not well-understood. We aimed to examine the role of Heg in mammalian liver development and functional maintenance.

Methods

Global (Heg^-/-) or liver endothelial cell (EC)-specific deletion of Heg (Lyve1-Cre;Heg^fl/fl ) mice were used to study the in vivo function of Heg in the liver. Carbon-ink anterograde and retrograde injection were used to visualize the 3-dimensional patterning of liver portal and biliary networks, respectively. RNA sequencing, histology, and molecular and biochemical assays were used to assess liver gene expression, protein distribution, liver inj

SUBMITTER: Zhu S 

PROVIDER: S-EPMC9059100 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature