Parallel G-quadruplex-mediated protein dimerization and activation.

ABSTRACT: We studied parallel G4-mediated protein dimerization and activation by incorporating a RHAU peptide with a fluorescent protein FRET pair CFP/YFP and an apoptotic casp9. Occurrence of energy tranfer (from donor CFP to acceptor YFP) and enhancement of 60-fold cleavage efficiency of casp9 were observed in the presence of parallel G4, which indicated that parallel G4 can induce dimerization and activation of proteins. This novel approach holds a great promise for studying G4-targeting functional dimeric proteins in celllular biology.

SUBMITTER: Thi Truong TT 

PROVIDER: S-EPMC9059161 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature