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Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells.

ABSTRACT: We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M^-1 s^-1, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials.

SUBMITTER: Cai X

PROVIDER: S-EPMC9059488 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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Tetra-fluorinated aromatic azide for highly efficient bioconjugation in living cells.

Cai Xuekang X Wang Dan D Gao Yasi Y Yi Long L Yang Xing X Xi Zhen Z

RSC advances 20181219 1

We developed a fast strain-promoted azide-alkyne cycloaddition reaction (SPAAC) by tetra-fluorinated aromatic azide with a kinetic constant of 3.60 M<sup>-1</sup> s<sup>-1</sup>, which is among the fastest SPAAC ligations reported so far. We successfully employed the reaction for covalent labelling of proteins with high efficiency and for bioimaging of mitochondria in living cells. The reaction could be a generally useful toolbox for chemical biology and biomaterials. ...[more]

PMID: 35521584

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