Expression of N-Terminal Cysteine Aβ₄₂ and Conjugation to Generate Fluorescent and Biotinylated Aβ₄₂.

ABSTRACT: Fluorescent derivatives of the β-amyloid peptides (Aβ) are valuable tools for studying the interactions of Aβ with cells. Facile access to labeled expressed Aβ offers the promise of Aβ with greater sequence and stereochemical integrity, without impurities from amino acid deletion and epimerization. Here, we report methods for the expression of Aβ₄₂ with an N-terminal cysteine residue, Aβ_(C1-42), and its conjugation to generate Aβ₄₂ bearing fluorophores or biotin. The methods rely on the hitherto unrecognized observation that expression of the Aβ_(MC1-42) gene yields the Aβ_(C1-42) peptide, because the N-terminal methionine is endogenously excised by Escherichia coli. Conjugation of Aβ_(C1-42) with maleimide-functionalize

SUBMITTER: Zhang S 

PROVIDER: S-EPMC9059633 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature