Highly efficient redox reaction between potassium permanganate and 3,3',5,5'-tetramethylbenzidine for application in hydrogen peroxide based colorimetric assays.

ABSTRACT: Potassium permanganate (KMnO₄) is one of the most important oxidants, which plays important roles in many fields. Here, we found that KMnO₄ could directly induce the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to generate an oxidized product with a color change. This redox reaction is highly efficient, and 1 μM KMnO₄ is enough to cause detectable changes in the absorbance signal. Meanwhile, this reaction is very fast and the generated blue product can stabilize for a relatively long period, which has great advantages in practical applications. Since hydrogen peroxide (H₂O₂) is able to react with KMnO₄ under acidic conditions, the KMnO₄-TMB system can be used for the detection of H₂O₂; t

SUBMITTER: Sun Y 

PROVIDER: S-EPMC9059746 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature