Nucleic acid functionalized fiber optic probes for sensing in evanescent wave: optimization and application.

ABSTRACT: Nucleic acid functionalized evanescent wave fiber optic (EWFO) biosensors have attracted much attention due to their remarkable advantages in both device configuration and sensing performance. One critical technique in EWFO biosensor fabrication is its surface modification, which requires (1) minimal nonspecific adsorption and (2) high-quality DNA immobilization to guarantee satisfactory sensing performances. Focusing on these two requirements, a series of optimizations have been conducted in this work to develop reliable DNA-functionalized EWFO probes. Firstly, the surface planeness of EWFO probes were found to be greatly improved by a novel HF/HNO₃ mixture etching solution. Both atomic force microscopy (AFM) and X-ray photoelectron spectroscopy (XPS) were conducted to investig

SUBMITTER: Zhu X 

PROVIDER: S-EPMC9059834 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature