Dataset Information

Genetic Characterization of bla _NDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98.

ABSTRACT:

Purpose

NDM-1-producing Citrobacter portucalensis and Citrobacter freundii simultaneously occurred in a hospital. This study aims to characterize the bla _NDM-1-carrying plasmids in these Citrobacter strains.

Methods

Cf7303, Cf7308, and Cf7313 were recovered from three patients in a teaching hospital from September 24 to October 1, 2021. Bacteria were identified by MALDI-TOF mass spectrometry, and antibiotics susceptibility tests were determined by VITEK^® 2 compact system. Whole-genome sequencing (WGS) was performed using the HiSeq Illumina and QNome platform to characterize the genomes.

Results

Cf7303 was identified as C. portucalensis Sequence Type 328 by WGS, and harbored two plasmids, namely pCf7303 and a novel IncFIB pNDM-Cf7303 on which antibiotic-resistant genes (bla _TEM-1, bla _CTX-M-14, bla _NDM-1, aac (3)-IId, aadA2, fosA3, sul1, sul2, catA2, tetD, dfrA12, qacEdelta1, mph(A), and ble _MBL) are located. C. freundii strain Cf7308 and Cf7313 belonged to the same Sequence Type 98. Cf7308 contained two plasmids, pCf7308, and an IncN1 pNDM-Cf7308 with homology to pNDM-BTR in E. coli and pNDM-CWH001 in C. freundii.

Conclusion

We characterized a putatively novel IncFIB plasmid carrying bla _NDM-1 in C. portucalensis. In addition, the closely related bla _NDM-1-carrying IncN1 plasmids in E. coli and C. freundii suggest that interspecies or intraspecies horizontal transfer occurs in China.

SUBMITTER: Wang L

PROVIDER: S-EPMC9059874 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Genetic Characterization of <i>bla</i> <sub>NDM-1</sub>-Carrying <i>Citrobacter portucalensis</i> Sequence Type 328 and <i>Citrobacter freundii</i> Sequence Type 98.

Wang Lijun L Li Ziyao Z Xiao Nan N Tang Jie J He Yu Y Guo Jun J Zhao Xiuying X

Infection and drug resistance 20220428

<h4>Purpose</h4>NDM-1-producing <i>Citrobacter portucalensis</i> and <i>Citrobacter freundii</i> simultaneously occurred in a hospital. This study aims to characterize the <i>bla</i> <sub>NDM-1-</sub>carrying plasmids in these <i>Citrobacter</i> strains.<h4>Methods</h4>Cf7303, Cf7308, and Cf7313 were recovered from three patients in a teaching hospital from September 24 to October 1, 2021. Bacteria were identified by MALDI-TOF mass spectrometry, and antibiotics susceptibility tests were determin ...[more]

PMID: 35510156

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Dataset Information

Genetic Characterization of bla _NDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98.

Purpose

Methods

Results

Conclusion

Publications

Genetic Characterization of <i>bla</i> <sub>NDM-1</sub>-Carrying <i>Citrobacter portucalensis</i> Sequence Type 328 and <i>Citrobacter freundii</i> Sequence Type 98.

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Dataset Information

Genetic Characterization of bla NDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98.

Purpose

Methods

Results

Conclusion

Publications

Genetic Characterization of <i>bla</i> <sub>NDM-1</sub>-Carrying <i>Citrobacter portucalensis</i> Sequence Type 328 and <i>Citrobacter freundii</i> Sequence Type 98.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Genetic Characterization of bla _NDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98.