Quantification of azide groups on a material surface and a biomolecule using a clickable and cleavable fluorescent compound.

ABSTRACT: Here, we propose a novel method for quantifying azide groups on a solid surface and a protein using a clickable and cleavable fluorescent compound. The clickable and cleavable fluorescent compound conjugates with the azide groups on the material surface and the fluorophore is then liberated into the solvent via a cleavage reaction, which can be simply quantified with a conventional fluorometer.

SUBMITTER: Sakai R 

PROVIDER: S-EPMC9060625 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature