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Photoswitchable probe with distinctive characteristics for selective fluorescence imaging and long-term tracing.

ABSTRACT: A photo-switchable and high-contrast bio-imaging indicator 4,4'-(1E,1'E)-(4,4'-(cyclopentene-1,2-diyl)bis(5-methylthiophene-4,2-diyl))bis(methan-1-yl-1-ylidene)bis(azan-1-yl-1-ylidene)bis(2-(benzo[d]thiazol-2-yl)phenol) (BMBT) has been demonstrated, by integrating photochromophore with excited-state intramolecular proton transfer (ESIPT) moiety. The ability of reversible emission switching enables arbitrarily selective labeling or concealing of cells simply by controlling light irradiation. Besides, when the emission was switched on, BMBT is demonstrated to exhibit unique characteristics of aggregation induced emission (AIE), providing a high on-off ratio for favorable bio-imaging. Thus, the non-labeling and easily-controlled selective imaging, as well as good biocompatibility indicates BMBT to be a favorable cell probe with great potentials for functional bio-imaging fluorophore.

SUBMITTER: Tu Z

PROVIDER: S-EPMC9060663 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Photoswitchable probe with distinctive characteristics for selective fluorescence imaging and long-term tracing.

Tu Zhenzhen Z Zhang Qian Q Xu Xiuxia X Wang Lianhui L Qian Yan Y Huang Wei W

RSC advances 20190207 9

A photo-switchable and high-contrast bio-imaging indicator 4,4'-(1E,1'E)-(4,4'-(cyclopentene-1,2-diyl)bis(5-methylthiophene-4,2-diyl))bis(methan-1-yl-1-ylidene)bis(azan-1-yl-1-ylidene)bis(2-(benzo[d]thiazol-2-yl)phenol) (BMBT) has been demonstrated, by integrating photochromophore with excited-state intramolecular proton transfer (ESIPT) moiety. The ability of reversible emission switching enables arbitrarily selective labeling or concealing of cells simply by controlling li ...[more]

PMID: 35514639

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