Determination of sialic acid in serum samples by dispersive solid-phase extraction based on boronate-affinity magnetic hollow molecularly imprinted polymer sorbent.

ABSTRACT: Boronate-affinity magnetic hollow molecularly imprinted polymers (B-MhMIPs) were prepared with sialic acid (SA) as the template, 3-aminophenylboronic acid (APBA) as the functional monomer and glycidilmethacrylate (GMA) as the co-monomer to chemisorb Fe₃O₄ nanoparticles. Furthermore, the hollow structure made the nanoparticles have more binding sites at both internal and external surfaces, which can facilitate the removal of template molecules from polymers and enhance the adsorption abilities towards SA. After optimizing, the adsorption pH was controlled at 4.0, and this was different from most cis-diol-containing compounds. Under the optimal conditions, the limit of detection for SA was 0.025 μg mL^-1 (n = 3). This method was applied to analyz

SUBMITTER: Huang W 

PROVIDER: S-EPMC9060700 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature