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New rhodamine B-based chromo-fluorogenic probes for highly selective detection of aluminium(iii) ions and their application in living cell imaging.

ABSTRACT: Two rhodamine B-based fluorescent probes, BOS1 and BOS2, were designed and synthesized with good yields via the condensation reactions between the o-diaminobenzene modified rhodamine core structure (RBO) and salicylaldehyde derivatives. Both the probes exhibited remarkable absorbance-on and fluorescence-on responses to Al³⁺ over other metal ions in ethanol-water (1 : 9, v/v) medium via the rhodamine ring-opening approach, which can be used for "naked-eye" Al³⁺ detection over a broad pH range (5-9). The fluorescence intensities of the probes were linear with the Al³⁺ ion concentration, resulting in a low limit of detection of 1.839 μM (BOS1) and 1.374 μM (BOS2) for Al³⁺. In addition, the MTT assays and cell imaging experiments of Al³⁺ in SGC-7901 living cells demonstrated that the probes had negligible cytotoxicity, and were cell permeable and suitable for sensing Al³⁺ in biological systems.

SUBMITTER: Leng X

PROVIDER: S-EPMC9060861 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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New rhodamine B-based chromo-fluorogenic probes for highly selective detection of aluminium(iii) ions and their application in living cell imaging.

Leng Xin X Xu Wenfeng W Qiao Chengfang C Jia Xu X Long Ying Y Yang Bingqin B

RSC advances 20190219 11

Two rhodamine B-based fluorescent probes, BOS1 and BOS2, were designed and synthesized with good yields via the condensation reactions between the o-diaminobenzene modified rhodamine core structure (RBO) and salicylaldehyde derivatives. Both the probes exhibited remarkable absorbance-on and fluorescence-on responses to Al3+ over other metal ions in ethanol-water (1 : 9, v/v) medium via the rhodamine ring-opening approach, which can be used for "naked-eye" Al3 ...[more]

PMID: 35517253

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Project description:The synthesis of capped mesoporous silica nanoparticles (MSN) conjugated with an antibody (AB) as a gatekeeper has been carried out in order to obtain a delivery system able to release an entrapped cargo (dye) in the presence of a target molecule (antigen) to which the conjugated antibody binds selectively. In particular, MSN loaded with rhodamine B and functionalized on the external surface with a suitable derivative of N-(t-butyl)-3-oxo-(5α,17β)-4-aza-androst-1-ene-17-carboxamide (finasteride) have been prepared (S1). The addition of polyclonal antibodies against finasteride induced capping of the pores due to the interaction with the anchored hapten-like finasteride derivative to give a MSN-hapten-AB nanoparticle S1-AB. It was found that the addition of capped material S1-AB to water solutions containing finasteride resulted in displacement of the antibody, pore uncapping and entrapped-dye release. The response of the gated material is highly selective, and only finasteride, among other steroids, was able to induce a significant uncapping process. Compared with finasteride, the finasteride metabolite was able to release 17 % of the dye, whereas the exogen steroids testosterone, metenolone and 16-β-hydroxystanozolol only induced very little release of rhodamine B (lower than 10 %) from aqueous suspensions containing sensing solid S1-AB. A detection limit as low as 20 ppb was found for the fluorimetric detection of finasteride. In order to evaluate a possible application of the material for label-free detection of finasteride, the capped material was isolated and stored to give final sensing solid S1-AB-i. It was found to display a similar behavior towards finasteride as to that shown by freshly prepared S1-AB; even after a period of two months, no significant loss of selectivity or sensitivity was noted. Moreover, to study the application for the detection of finasteride in biological samples, this "aged" material, S1-AB-i, was tested using commercially available blank urine as matrix. Samples containing 70 and 90 % blank urine were spiked with a defined amount of finasteride, and the concentration was determined using capped S1-AB-i. Recovery ranges from 94 % to 118 % were reached.

Dataset Information

New rhodamine B-based chromo-fluorogenic probes for highly selective detection of aluminium(iii) ions and their application in living cell imaging.

Publications

New rhodamine B-based chromo-fluorogenic probes for highly selective detection of aluminium(iii) ions and their application in living cell imaging.

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