A colorimetric and ratiometric fluorescent sensor for sequentially detecting Cu²⁺ and arginine based on a coumarin-rhodamine B derivative and its application for bioimaging.

ABSTRACT: In this work, a colorimetric and ratiometric fluorescent sensor based on a coumarin-rhodamine B hybrid for the sequential recognition of Cu²⁺ and arginine (Arg) via the FRET mechanism was designed and synthesized. With the addition of Cu²⁺, the solution displayed a colorimetric change from pale yellow to pink which is discernible by the naked eye. Additionally, the fluorescence intensities of the sensor exhibited ratiometric changes for the detection of Cu²⁺ at 490 and 615 nm under a single excitation wavelength of 350 nm, which corresponded to the emissions of coumarin and rhodamine B moieties, respectively. The fluorescence color change could be visualized from blue to pink. The limits of detection were determined to be as low as 0.50 and 0.47 μM f

SUBMITTER: Wang S 

PROVIDER: S-EPMC9060912 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature