The m⁶A demethylase FTO promotes the osteogenesis of mesenchymal stem cells by downregulating PPARG.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most abundant posttranscriptional methylation modification that occurs in mRNA and modulates the fine-tuning of various biological processes in mammalian development and human diseases. In this study we investigated the role of m⁶A modification in the osteogenesis of mesenchymal stem cells (MSCs), and the possible mechanisms by which m⁶A modification regulated the processes of osteoporosis and bone necrosis. We performed systematic analysis of the differential gene signatures in patients with osteoporosis and bone necrosis and conducted m⁶A-RNA immunoprecipitation (m⁶A-RIP) sequencing to identify the potential regulatory genes involved in osteogenesis. We showed that fat mass and obesity (FTO)

SUBMITTER: Chen LS 

PROVIDER: S-EPMC9061799 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature