Nanobody-based label-free photoelectrochemical immunoassay for highly sensitive detection of SARS-CoV-2 spike protein.

ABSTRACT: Until now, COVID-19 caused by SARS-CoV-2 is engulfing the worldwide and still ranging to date, continuing to threaten the public health. The main challenge facing COVID-19 epidemic is short of fast-response and high-efficiency methods to determine SARS-CoV-2 viral pathogens. Herein, a nanobody-based label-free photoelectrochemical (PEC) immunosensor has been fabricated for rapidly detecting SARS-CoV-2 spike protein. As a small-size and high-stability antibody, nanobody was directly and well immobilized with Au nanoparticles and TiO₂ spheres by the interaction. Au deposited TiO₂ nanomaterial possessed 8.5 times photoelectric performance in comparison with TiO₂ in the presence of electron donor owing to surface plasma resonance effect of Au. Based on the ster

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9062376 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature