Characterization of the Secretome, Transcriptome, and Proteome of Human β Cell Line EndoC-βH1.

ABSTRACT: Early diabetes research is hampered by limited availability, variable quality, and instability of human pancreatic islets in culture. Little is known about the human β cell secretome, and recent studies question translatability of rodent β cell secretory profiles. Here, we verify representativeness of EndoC-βH1, one of the most widely used human β cell lines, as a translational human β cell model based on omics and characterize the EndoC-βH1 secretome. We profiled EndoC-βH1 cells using RNA-seq, data-independent acquisition, and tandem mass tag proteomics of cell lysate. Omics profiles of EndoC-βH1 cells were compared to human β cells and insulinomas. Secretome composition was assessed by data-independent acquisition proteomics. Agreement between EndoC-βH1 cells and primary adult human β ce

SUBMITTER: Ryaboshapkina M 

PROVIDER: S-EPMC9062487 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature