High-Throughput 3D Phenotyping of Plant Shoot Apical Meristems From Tissue-Resolution Data.

ABSTRACT: Confocal imaging is a well-established method for investigating plant phenotypes on the tissue and organ level. However, many differences are difficult to assess by visual inspection and researchers rely extensively on ad hoc manual quantification techniques and qualitative assessment. Here we present a method for quantitatively phenotyping large samples of plant tissue morphologies using triangulated isosurfaces. We successfully demonstrate the applicability of the approach using confocal imaging of aerial organs in Arabidopsis thaliana. Automatic identification of flower primordia using the surface curvature as an indication of outgrowth allows for high-throughput quantification of divergence angles and further analysis of individual flowers. We demonstrate the throughput o

SUBMITTER: Ahl H 

PROVIDER: S-EPMC9062647 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature