Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk.

ABSTRACT: Two exopolysaccharides (EPS) producing strains, isolated from raw donkey milk were identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5 using 16S rDNA sequencing. The Box Benheken design exhibited the highest yield of EPS-SL (672.342 mg/L) produced by SL and of EPS-N5 (901 mg/L) produced by N5. The molecular weight was 1.68×10⁴ for EPS-SL and 1.55×10⁴ Da for EPS-N5. FTIR, NMR and GC-MS analysis showed that the EPS are heteropolysaccharides. The SEM image showed that the EPS-SL was smooth and represented a lotus leaf shape and EPS-N5 revealed a stiff-like, porous appearance and was more compact than EPS-SL. The TGA analyses showed high thermal stability and degradation temperature. Additionally, the two EPSs possessed antibacterial a

SUBMITTER: Derdak R 

PROVIDER: S-EPMC9062669 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature