Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens.

ABSTRACT:

Objectives

Formalin-fixed paraffin-embedded (FFPE) tissue is the standard material for diagnostic pathology but poses relevant hurdles to accurate protein extraction due to cross-linking and chemical alterations. While numerous extraction protocols and chemicals have been described, systematic comparative analyses are limited. Various parameters were thus investigated in their qualitative and quantitative effects on protein extraction (PE) efficacy. Special emphasis was put on preservation of membrane proteins (MP) as key subgroup of functionally relevant proteins.

Methods

Using the example of urothelial carcinoma, FFPE tissue sections were subjected to various deparaffinization, protein extraction and antigen retrieval protocols and buffers as well as different extraction t

SUBMITTER: Dressler FF 

PROVIDER: S-EPMC9063121 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature