DNA methylation of miR-138 regulates cell proliferation and EMT in cervical cancer by targeting EZH2.

ABSTRACT:

Background

Emerging evidence has identified miR-138 as a tumor suppressor that can suppress the proliferation of various cancers. Meanwhile, the cause of abnormal miR-138 expression in cervical cancer remains uncertain. This study clarified the mechanism by which miR-138 regulates proliferation, invasion, metastasis, and EMT in cervical cancer cells.

Results

miR-138 expression in human cervical cancer and adjacent normal tissue was measured using qPCR. SiHa and C33A cells were used to determine the function of miR-138 via miR-138 mimic or inhibitor transfection, followed by wound healing, Cell Counting Kit-8, flow cytometry, and Transwell assays. Epithelial and mesenchymal marker expression was analyzed using Western blotting. DNA methylation in the miR-138 promoter was exam

SUBMITTER: Chen R 

PROVIDER: S-EPMC9063191 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature