An RNA-sequencing transcriptome of the rodent Schwann cell response to peripheral nerve injury.

ABSTRACT:

Background

The important contribution of glia to mechanisms of injury and repair of the nervous system is increasingly recognized. In stark contrast to the central nervous system (CNS), the peripheral nervous system (PNS) has a remarkable capacity for regeneration after injury. Schwann cells are recognized as key contributors to PNS regeneration, but the molecular underpinnings of the Schwann cell response to injury and how they interact with the inflammatory response remain incompletely understood.

Methods

We completed bulk RNA-sequencing of Schwann cells purified acutely using immunopanning from the naïve and injured rodent sciatic nerve at 3, 5, and 7 days post-injury. We used qRT-PCR and in situ hybridization to assess cell purity and probe dataset integrity. Finally, we

SUBMITTER: Brosius Lutz A 

PROVIDER: S-EPMC9063194 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature