Dataset Information

PICALM exerts a role in promoting CRC progression through ERK/MAPK signaling pathway.

ABSTRACT:

Background

Colorectal cancer (CRC) is a common malignant tumor in gastrointestinal tract with high incidence and mortality. In this study, the functions and potential mechanism of phosphatidylinositol-binding clathrin assembly protein (PICALM) in CRC were preliminarily explored.

Methods

Based on the Cancer Genome Atlas database and immunohistochemistry staining, revealing that the expression level of PICALM in CRC tissues was higher than that in adjacent normal tissues.

Results

Moreover, loss-of-function and gain-of-function assays in HCT 116 and RKO cells found that PICALM promotes proliferation and migration of CRC cells and inhibits apoptosis. Consistently, knockdown of PICALM inhibited tumorigenicity of CRC cells in vivo. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that knockdown of PICALM resulted in the enrichment of MAPK signaling pathway. Treatment of CRC cells with MAPK inhibitor reversed the effects of PICALM overexpression on proliferation and apoptosis. In addition, overexpression of PICALM upregulated the protein levels of ERK1/2 (p-ERK1/2), MEK1/2 (p-MEK1/2), p38 (p-p38) and JNK (p-JNK), and these effects were partially alleviated by the treatment of MAPK inhibitor.

Conclusions

In summary, the study presented the new discovery that PICALM promoted CRC progression through ERK/MAPK signaling pathway, which drew further interest regarding its clinical application as a promising therapeutic target.

SUBMITTER: Zhang X

PROVIDER: S-EPMC9063212 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

PICALM exerts a role in promoting CRC progression through ERK/MAPK signaling pathway.

Zhang Xitao X Liu Tianlai T Huang Jinlin J He Jianping J

Cancer cell international 20220502 1

<h4>Background</h4>Colorectal cancer (CRC) is a common malignant tumor in gastrointestinal tract with high incidence and mortality. In this study, the functions and potential mechanism of phosphatidylinositol-binding clathrin assembly protein (PICALM) in CRC were preliminarily explored.<h4>Methods</h4>Based on the Cancer Genome Atlas database and immunohistochemistry staining, revealing that the expression level of PICALM in CRC tissues was higher than that in adjacent normal tissues.<h4>Results ...[more]

PMID: 35501863

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Dataset Information

PICALM exerts a role in promoting CRC progression through ERK/MAPK signaling pathway.

Background

Methods

Results

Conclusions

Publications

PICALM exerts a role in promoting CRC progression through ERK/MAPK signaling pathway.

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