Chromosomal localization of cohesin is differentially regulated by WIZ, WAPL, and G9a.

ABSTRACT:

Background

The cohesin complex is essential for proper chromosome structure and gene expression. Defects in cohesin subunits and regulators cause changes in cohesin complex dynamics and thereby alter three-dimensional genome organization. However, the molecular mechanisms that drive cohesin localization and function remain poorly understood.

Results

In this study, we observe that loss of WIZ causes changes to cohesin localization that are distinct from loss of the known WIZ binding partner G9a. Whereas loss of WIZ uniformly increases cohesin levels on chromatin at known binding sites and leads to new, ectopic cohesin binding sites, loss of G9a does not. Ectopic cohesin binding on chromatin after the loss of WIZ occurs at regions that are enriched for activating histone modif

SUBMITTER: Justice M 

PROVIDER: S-EPMC9063240 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature