Tolerance against butanol stress by disrupting succinylglutamate desuccinylase in Escherichia coli.

ABSTRACT: Background: The four-carbon alcohol, butanol, is emerging as a promising biofuel and efforts have been undertaken to improve several microbial hosts for its production. However, most organisms have very low tolerance to n-butanol (up to 2% (v/v)), limiting the economic viability of butanol production. Although genomic tools (transcriptomics, proteomics, and metabolomics) have been widely used to investigate the cellular response to butanol stress, the existing knowledge of the molecular mechanisms involved in butanol tolerance is limited, and strain improvement is difficult due to the complexity of the regulatory network. Results: In this study, a butanol-tolerant Escherichia coli was constructed by disrupting gene astE (encoding succinylglutamate desucci

SUBMITTER: Guo Y 

PROVIDER: S-EPMC9063396 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature