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Phosphorescent immunosensor for simple and sensitive detection of microcystin-LR in water.

ABSTRACT: A simple and sensitive Mn-ZnS quantum dot room-temperature phosphorescent immunosensor for detecting microcystin-LR was developed. This sensor adopted antigens and antibodies as recognition units and used Mn-ZnS RTP QDs as sensing materials to specifically bind with MC-LR. The structurally specific binding between the microcystin-LR antibody and MC-LR led to the aggregation of antibody-crosslinked QDs, and then the electrons of QDs would be transferred to the complex, leading to the phosphorescence quenching of QDs. The microcystin-LR antigen-antibody specific binding site was first analyzed. This phosphorescent immunosensor rapidly and sensitively detected microcystin-LR, with linear ranges of 0.2-1.5 μg L^-1 and 1.5-20 μg L^-1 and a detection limit of up to 0.024 μg L^-1. Meanwhile, coexisting pollutants of microcystin-LR in water did not significantly interfere with microcystin-LR detection. The new sensor was applied to detect real water samples and showed high sensitivity and selectivity.

SUBMITTER: Qin J

PROVIDER: S-EPMC9063644 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Phosphorescent immunosensor for simple and sensitive detection of microcystin-LR in water.

Qin Jin J Sun Xiaojie X Li Dongxia D Yan Guiqin G

RSC advances 20190425 22

A simple and sensitive Mn-ZnS quantum dot room-temperature phosphorescent immunosensor for detecting microcystin-LR was developed. This sensor adopted antigens and antibodies as recognition units and used Mn-ZnS RTP QDs as sensing materials to specifically bind with MC-LR. The structurally specific binding between the microcystin-LR antibody and MC-LR led to the aggregation of antibody-crosslinked QDs, and then the electrons of QDs would be transferred to the complex, leading to the phosphoresce ...[more]

PMID: 35515855

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