Protein-engineered scaffolds for in vitro 3D culture of primary adult intestinal organoids.

ABSTRACT: Though in vitro culture of primary intestinal organoids has gained significant momentum in recent years, little has been done to investigate the impact of microenvironmental cues provided by the encapsulating matrix on the growth and development of these fragile cultures. In this work, the impact of various in vitro culture parameters on primary adult murine organoid formation and growth are analyzed with a focus on matrix properties and geometric culture configuration. The air-liquid interface culture configuration was found to result in enhanced organoid formation relative to a traditional submerged configuration. Additionally, through use of a recombinantly engineered extracellular matrix (eECM), the effects of biochemical and biomechanical cues were independently studied. Decreasing me

SUBMITTER: DiMarco RL 

PROVIDER: S-EPMC9063856 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature